pbmcs isolated by density gradient centrifugation Search Results


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Biochrom mononuclear cells isolation by density gradient centrifugation with ficoll
Plate-stabilized and filled femoral defect ( a ). A 5 mm critical-size defect in the right femur of a rat is stabilized with a 5-hole plate. The gap is filled with demineralized bone matrix fibers (f-DBM) loaded with BMCs. Macroscopic image of the fibrous tissue scaffold ( b ). Fluorescence microscopic image; adherent CFSE-stained BMCs (green dots, marked with red arrow) can be seen at different depth levels of the f-DBM plexus (original magnification 50×) ( c ). FACS characterization of rat BMCs ( d ). Mainly <t>mononuclear</t> cell types (lymphoid and monocytic cells) remain after density gradient centrifugation.
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Plate-stabilized and filled femoral defect ( a ). A 5 mm critical-size defect in the right femur of a rat is stabilized with a 5-hole plate. The gap is filled with demineralized bone matrix fibers (f-DBM) loaded with BMCs. Macroscopic image of the fibrous tissue scaffold ( b ). Fluorescence microscopic image; adherent CFSE-stained BMCs (green dots, marked with red arrow) can be seen at different depth levels of the f-DBM plexus (original magnification 50×) ( c ). FACS characterization of rat BMCs ( d ). Mainly <t>mononuclear</t> cell types (lymphoid and monocytic cells) remain after density gradient centrifugation.
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ICN Biomedicals mononuclear cells isolated by density gradient centrifugation
Plate-stabilized and filled femoral defect ( a ). A 5 mm critical-size defect in the right femur of a rat is stabilized with a 5-hole plate. The gap is filled with demineralized bone matrix fibers (f-DBM) loaded with BMCs. Macroscopic image of the fibrous tissue scaffold ( b ). Fluorescence microscopic image; adherent CFSE-stained BMCs (green dots, marked with red arrow) can be seen at different depth levels of the f-DBM plexus (original magnification 50×) ( c ). FACS characterization of rat BMCs ( d ). Mainly <t>mononuclear</t> cell types (lymphoid and monocytic cells) remain after density gradient centrifugation.
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Corning Life Sciences pbmcs isolated by ficoll-hypaque density gradient centrifugation
Plate-stabilized and filled femoral defect ( a ). A 5 mm critical-size defect in the right femur of a rat is stabilized with a 5-hole plate. The gap is filled with demineralized bone matrix fibers (f-DBM) loaded with BMCs. Macroscopic image of the fibrous tissue scaffold ( b ). Fluorescence microscopic image; adherent CFSE-stained BMCs (green dots, marked with red arrow) can be seen at different depth levels of the f-DBM plexus (original magnification 50×) ( c ). FACS characterization of rat BMCs ( d ). Mainly <t>mononuclear</t> cell types (lymphoid and monocytic cells) remain after density gradient centrifugation.
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Image Search Results


Plate-stabilized and filled femoral defect ( a ). A 5 mm critical-size defect in the right femur of a rat is stabilized with a 5-hole plate. The gap is filled with demineralized bone matrix fibers (f-DBM) loaded with BMCs. Macroscopic image of the fibrous tissue scaffold ( b ). Fluorescence microscopic image; adherent CFSE-stained BMCs (green dots, marked with red arrow) can be seen at different depth levels of the f-DBM plexus (original magnification 50×) ( c ). FACS characterization of rat BMCs ( d ). Mainly mononuclear cell types (lymphoid and monocytic cells) remain after density gradient centrifugation.

Journal: Cells

Article Title: Fibrous Demineralized Bone Matrix (DBM) Improves Bone Marrow Mononuclear Cell (BMC)-Supported Bone Healing in Large Femoral Bone Defects in Rats

doi: 10.3390/cells10051249

Figure Lengend Snippet: Plate-stabilized and filled femoral defect ( a ). A 5 mm critical-size defect in the right femur of a rat is stabilized with a 5-hole plate. The gap is filled with demineralized bone matrix fibers (f-DBM) loaded with BMCs. Macroscopic image of the fibrous tissue scaffold ( b ). Fluorescence microscopic image; adherent CFSE-stained BMCs (green dots, marked with red arrow) can be seen at different depth levels of the f-DBM plexus (original magnification 50×) ( c ). FACS characterization of rat BMCs ( d ). Mainly mononuclear cell types (lymphoid and monocytic cells) remain after density gradient centrifugation.

Article Snippet: The bone marrow was diluted with PBS (1:3), and mononuclear cells were isolated by density gradient centrifugation with Ficoll (1.077 g/cm 3 , Biochrom, Berlin, Germany) at 800 g for 20 min without break at room temperature [ ].

Techniques: Fluorescence, Staining, Gradient Centrifugation